LC-MS, GC-MS, and NMR Spectroscopic Analysis of Withania somnifera (Ashwagandha) Root Extract After Treatment with the Energy of Consciousness (The Trivedi Effect®)
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
Aileen Carol Lee,
Aksana Hancharuk,
Carola Marina Sand,
Debra Jane Schnitzer,
Rudina Thanasi,
Eileen Mary Meagher,
Faith Ann Pyka,
Gary Richard Gerber,
Johanna Catharina Stromsnas,
Judith Marian Shapiro,
Laura Nelson Streicher,
Lorraine Marie Hachfeld,
Matthew Charles Hornung,
Patricia M. Rowe,
Sally Jean Henderson,
Sheila Maureen Benson,
Shirley Theresa Holmlund,
Stephen P. Salters,
Parthasarathi Panda,
Snehasis Jana
Issue:
Volume 5, Issue 2, April 2017
Pages:
27-37
Received:
6 February 2017
Accepted:
18 February 2017
Published:
9 March 2017
Abstract: Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.35, 5.55, 5.94, 6.25, 6.63, 6.76, 7.92, 8.04, 8.60, 8.73, and 9.31 min were significantly reduced by 6.15% to 60.67% compared with the control sample at Rt of 5.43, 5.65, 5.95, 6.29, 6.76, 6.85, 8.03, 8.14, 8.68, 8.78, and 9.30 min. Consequently, the peak area% of the treated sample at Rt of 7.25, 7.30, 8.27, and 8.47 min were significantly increased by 26.32%, 7.99%, 16.93% and 7.97% compared with the control sample at Rt of 7.37, 7.41, 8.36, and 8.55 min, respectively. A total of 13 withanolides were proposed with their structure from the deduced molecular mass at m/z 470, 472, 488, 504, 782, and 991 through LC-MS, GC-MS, 1H and 13C NMR analysis of both the control and treated samples. Viscosa lactone B, 27-hydroxy withanolide A, (20S, 22R)-α, 6α-epoxy-β, 5β, 27-trihydroxy-1-oxowitha-24-enolide, (20S, 22R)-4β, 5β, 6α, 27-tetrahydroxy-1-oxo-with-2, 24-dienolide were proposed in the control and treated samples at Rt of 6.85 and 7.30 min, respectively. Dihydrowithanolide D was only identified in the control sample at Rt of 7.41 min, whereas withanoside IV or withanoside VI was only present in the Biofield Treated sample at Rt of 6.76 min. Withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide were found in both the control and treated samples. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.
Abstract: Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was d...
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Chromatographic and Spectroscopic Characterization of the Consciousness Energy Healing Treated Withania somnifera (Ashwagandha) Root Extract
Mahendra Kumar Trivedi,
Alice Branton,
Dahryn Trivedi,
Gopal Nayak,
William Dean Plikerd,
Peter L. Surguy,
Robert John Kock,
Rolando Baptista Piedad,
Russell Phillip Callas,
Sakina A. Ansari,
Sandra Lee Barrett,
Sara Friedman,
Steven Lee Christie,
Su-Mei Chen Liu,
Susan Elizabeth Starling,
Susan Jones,
Susan Mardis Allen,
Susanne Kathrin Wasmus,
Terry Ann Benczik,
Thomas Charles Slade,
Thomas Orban,
Victoria L. Vannes,
Victoria Margot Schlosser,
Yusif Sarkis Yamin Albino,
Kalyan Kumar Sethi,
Parthasarathi Panda,
Snehasis Jana
Issue:
Volume 5, Issue 2, April 2017
Pages:
38-47
Received:
20 March 2017
Accepted:
31 March 2017
Published:
8 May 2017
Abstract: Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic properties of the phytoconstituents present in the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control (without treatment), while another part was treated with the Consciousness Energy Healing Treatment remotely by twenty renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The liquid chromatographic data of the control and Biofield Energy Treated samples revealed that the retention time of the 25 chromatographic peaks remained same, whereas the peak area% i.e. the relative amount of the phytoconstituents was altered significantly. The peak area% of the treated ashwagandha root extract representing the phytoconstituents was significantly decreased in the range of 6.02% to 39.74% at Rt of 5.2, 5.3, 5.4, 5.6, 5.7, 6.9, 7.1, 7.3, 7.8, 7.9, 8.0, and 8.5 minutes compared to the control sample. On the contrary, the peak area% of the other phytoconstituents present in the treated sample was significantly increased in the range of 4.12% to 82.32% at Rt of 5.1, 6.4, 6.6, 6.8, 8.1, 8.2, 8.4, 8.6, 8.8, 8.9, 9.0, 9.1, and 9.2 minutes, respectively compared to the control sample. A total of 16 withanolides such as sitoindoside IX, viscosa lactone B, dihydrowithanolide D, withanolide A, withaferin A, ixocarpalactone A, withanolide sulfoxide, etc. were proposed from the molecular mass at m/z 605, 489, 473, 471, 505, and 992 at the retention times of 6.9, 7.1, 7.8, 8.2, 8.5, and 9.2 minutes with the help of LC-MS, GC-MS and NMR data of both the samples. Subsequently, the mass peak intensities of the treated sample were significantly changed in the range of -61.24% to 106.61% compared with the control sample at the same retention time. These findings suggest that Energy of Consciousness Healing Treatment could be advantageous for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of ashwagandha extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, arthritis, stress, cancer, diabetes, sexual disorders, aging, and other chronic infections.
Abstract: Withania somnifera (ashwagandha) root extract is a popular health supplement, with purported health benefits including prevention and treatment of various diseases, i.e. anxiety, stress, etc. The objective of this experiment was to evaluate the impact of Energy of Consciousness Healing Treatment (The Trivedi Effect®) on the characteristic propertie...
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