Volume 5, Issue 6, December 2017, Page: 104-111
Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis
Mosab Yahya Al-Nour, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Omdurman Islamic University, Khartoum, Sudan
Malik Suliman Mohamed, Department of Pharmaceutics, Faculty of Pharmacy, University of Khartoum, Khartoum, Sudan
Noha Ahmed Abd Alfadil, Department of Pharmaceutical Microbiology, Faculty of Pharmacy, University of Alneelain, Khartoum, Sudan
Hisham Nouraldayem Altayb, Department of Microbiology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan
Salah-Eldin Gumaa El-lzaki, Department of Epidemiology, Molecular Epidemiology Laboratory, Tropical Medicine Institute Research, Khartoum, Sudan
Mohamed Ahmed Hassan, Applied Bioinformatics Center, Africa City of Technology, Khartoum, Sudan
Received: Nov. 28, 2017;       Accepted: Dec. 19, 2017;       Published: Jan. 17, 2018
DOI: 10.11648/j.ejb.20170506.12      View  1711      Downloads  72
Abstract
Pseudomonas stutzeri is a valuable bacteria for understanding of the taxonomical and the phylogenetic relationships. The study of genetic relationships between organisms or genes is carried out by molecular phylogeny. We aim to study the relationships according to16S rRNA gene sequences between our samples and the closest strains from other countries over the world. To our knowledge, the phylogenetic studies between strains from Sudan with strains from other countries were not done before. A total of 140 currency notes in different denominations were collected randomly from several locations including hospitals, food sellers and transporters. From the collected notes, a total of 135 bacterial colonies were isolated and from them 14 isolates were identified as a Pseudomonas stutzeri. In the study, streaking plate method was used for the isolation of pure bacterial culture, Chelex 100 method was used for DNA extraction, conventional PCR was used for amplification of the targeted gene, agarose gel electrophoresis and various bioinformatics tools were used for nucleotide sequence analysis. The PCR products were sent for Macrogen Company-Netherlands for purification and nucleotide sequencing. After sequencing 3 samples were noisy, hence they were excluded. According to phylogenetic analysis, we found that the samples were closely related to strains from south-east Asia (Indonesia), east Asia (China), south-central Asia (Bangladesh), south Asia (India), north Africa (Tunisia) and south Europe (Italy and Greece). Despite the samples were from the same source (currency notes), we found that there is broad sequence variation between them.
Keywords
Pseudomonas stutzeri, Currency Notes, Molecular Phylogenetic, Khartoum, Sudan
To cite this article
Mosab Yahya Al-Nour, Malik Suliman Mohamed, Noha Ahmed Abd Alfadil, Hisham Nouraldayem Altayb, Salah-Eldin Gumaa El-lzaki, Mohamed Ahmed Hassan, Molecular Phylogenetic Study on Pseudomonas stutzeri Isolated from Currency Notes in Khartoum State, Sudan and Identified Via16s rRNA Gene Sequence Analysis, European Journal of Biophysics. Vol. 5, No. 6, 2017, pp. 104-111. doi: 10.11648/j.ejb.20170506.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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